FLA-3000G
A wide array of possibilities.

The FLA-3000G with its wide format Imaging Plate gives you the power to do more. Now you can quickly perform gene expression profiling using commercially available cDNA membranes up to 22x22cm in size. How quickly? A complete 23x40cm Imaging Plate can be scanned in only 6 minutes.

And the FLA-3000G doesn't stop there. It allows you to process samples labelled with either radioisotopes or fluorescent dyes - all within a single research tool. Its combination of two solid-state Second Harmonic Generation lasers (blue: 473nm and green: 532nm) and a He-Ne laser (red: 633nm) makes the FLA-3000G truly unique. For example, it is the only system that can not only efficiently detect and discriminate between Cy3™ and Cy5™, but also between Cy2™ and Cy5™ samples. Plus the FLA-3000G continues to push the boundaries of performance for a multifunctional scanner in speed, throughput, sensitivity and resolution. All that, and a 23cm wide format Imaging Plate.

FLA-3000G
Fluorescent detection of DNA:	EtBr-stained agarose gel Sample: Lambda DNA / Hind III	SYBR Green I-stained agarose gel Sample: Lambda DNA / Hind III

Capturing fluorescent images with exceptional laser technology. Capturing images of samples labelled with fluorescent dyes has never been easier. At the heart of our FLA-3000G lies an exclusive, patented Second Harmonic Generation (SHG) laser assembly. Its SHG532nm green laser allows for numerous applications to satisfy even the most demanding laboratories. For instance, the use of RITC, SYPRO^® Red, HNPP and Cy3™ is now a practical reality. The FLA-3000G also gives you speed. Images of 50µm , 100µm or 200µm pixel size, to a maximum of 23x40cm, can be obtained in 6 minutes. Smaller areas, of course, take even less time to scan.

The FLA-3000G can also be configured with just the SHG473nm laser and a He-Ne 633nm laser making it ideal for SYBR^® Green I and II, ethidium bromide, FITC, Phycoerythrin and Cy5™, not to mention alkaline phosphatase substrates such as AttoPhos™, ECL Plus™ and DDAO-phosphate.

Capturing radioisotopic images with proven, patented technology. Fujifilm had more than fifteen years of experience with phosphor Imaging Plate (IP) technology for medical radiography before they expanded its use to bioresearch.  And the FLA-3000G makes good use of this development.  The Imaging Plate in the 3000G is approximately 100 times more sensitive to emitted radiation than conventional x-ray film.  It reduces sample exposure times by as much as 90% and the amount of radioisotope needed for detection by as much as 90%.  What's more, Imaging Plates are easy to use.  They are reusable.  They do not require a dark room or chemicals, and they can be exposed at room temperature.

Imaging Plates have a flexible polyester base coated with highly dispersed barium fluorohalide phosphor crystals (BaF(Br,I):Eu²⁺). When a radioactively labelled sample is exposed to an IP, the energy from the sample is transferred to the phosphor crystals, and stored as trapped electrons.  In short, the electrons become trapped to form 'F-centres' in the BaF(Br,I) matrix and Eu²⁺ ions trap holes.  These F-centres have an absorption band at about 600nm.  So scanning the exposed IP with the Ne-He (633nm) laser releases the trapped electrons to the conduction band.  The trapped electrons recombine with the holes trapped by Eu²⁺, and photons at about 400 nm are released.  This process is known as photo-stimulated luminescence.   The blue light emitted is collected to produce the digitised image.

This Imaging Plate technology makes the FLA-3000G a fast and accurate means for quantitative analysis of all alpha, beta, and gamma-emitting isotopes (including tritium).   Add to this its sensitivity for neutron detection and the FLA-3000G is ideal for a broad spectrum of applications.  From gene array analysis to drug metabolism, the FLA-3000G does it all.

FLA-3000G
Chemifluorescent Southern blotting:	Detection with AttopPhos™ Sample: pBR328 / Bam HI	Detection with DDAO-phosphate 9H Sample: left 4 lanes Lambda DNA / Hind III right 4 lanes Lambda DNA / Eco RI+ Hind III

Three lasers are better than two. The FLA-3000G performs a wide array of imaging applications from fluorescence to photo-stimulated luminescence.  It's ideal for SYBR^® Green I and II, SYPRO^® Orange, ethidium bromide, FITC, Phycoerythrin and Cy5™.  It's fast.  You get six-minute scanning at even the highest resolution for areas as large as 23x40cm.  And that's just part of the story.   Our patented SHG laser is the only laser technology that efficiently detects and discriminates between Cy3™ and Cy5™, but also Cy2™ and Cy5™ labelled samples.  And light emitted from our SHG laser is coherent which means it's not dispersed when you use thick gels.  So, unlike with other light sources, you get exceptional image quality no matter how thick your gel.

Speed, image quality, flexibility and extraordinary capabilities make the FLA-3000G a remarkable research tool with much of the credit going to the revolutionary SHG Laser.   The laser diode assembly in the 3000G uses our newly developed, periodically poled MgO/LiNbO3 (PPMGLN) crystals as a wavelength transformer.  Inside the crystal, the direction of the dipole is periodically inverted.  Hundreds of inverted regions with a width of approximately 2.5µm are aligned within the 1mm thick crystal.  The wavelength of the secondary light beam is then freely controlled by varying the width of the poled regions.  The use of this new crystal along with other SHG technology led to the world's first blue solid-state laser diode.

FLA-3000G
Fluorescent detection of protein:	SYPRO® Orange-stained protein SDS-PAGE	FITC: Conventional SDS-PAGE of protein

FLA-3000G Specifications

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