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The junior is a small portable luminometer which furthers the fine tradition of Bethold luminometers. junior is especially designed for versatility:
The junior is designed for manual injection and can therefore be used for the measurement of all common glow type bioluminescent and chemiluminescent applications in research, diagnostics and food technology. The performance of the instrument is excellent. Even as a simple instrument without data evaluation it can be used for a variety of applications.
Reporter Gene Assays Nowadays there is a broad range of reporter gene assays which show a constant light output over minutes or even hours. Measurement of firefly luciferase, ß-galactosidase or ß-glucurinodase are common sensitive luminescent methods to study gene regulation. Even the kits using a second luminescent reaction for the standardisation of transfection efficiency, dual reporter gene assay, can be measured with the junior.
ATP Monitoring Due to the increasing availability of glow type ATP kits, based on the light generating firefly reaction, junior is the ideal instrument for the whole range of ATP-based assays. Using the CLS II ATP kit of Boehringer Mannheim (FRG), down to 1 fmole of ATP can be measured. Since all living cells contain ATP, in hygiene monitoring ATP bioluminescence is used to check with a fast and simple test whether surfaces are contaminated, eg with bacteria. Measured data are stored with the number of the test and its destination. The results can be transferred to the PC and implemented into the HACCP system of the company.
NAD(P)H Assays NAD(P)H can be easily measured using the light generating reaction of bacterial luciferases.
Enzyme Measurements Since ATP and NAD(P)H are among the most important substances in the metabolic system, a lot of different applications are correlated to the measurement of these compounds. By use of linked reaction systems a large variety of enzymatic activities can be measured.
Luminescent Immunoassays (LIA, ILMA, Luminescence ELISA) The ability to measure glow type luminescenct immunoassays also makes the junior suitable for in vitro diagnostic applications. Data evaluation with standard programs like Excel® is easy through using the Winterm data capture program.
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| Detector: ultra fast single photon counter with spectral sensitivity range of 380-630nm | Data Storage: storage for the last 2000 results |
| Sensitivity: 1 fmole of ATP using the ATP Bioluminescence Assay Kit CLS II (Boehringer Mannhein, FRG) | PC software: Winterm data capture program for easy data transfer and evaluation |
| Dynamic Range: more than 6 decades | Power Supply: 240v 50Hz, 115v 60Hz |
| Tube format: 12mm diameter, 47-75mm height; 15mm diameter, 47-75mm height | Batteries: rechargeable NiCad 1600mAh |
| Display: graphic display by backlit LED, 128x64 pixels | Dimensions: 150 x 280 x 170mm (WxDxH) |
| Interface: RS232C interface for data transfer to a PC | Weight: approx 2kg net |
| Software: integrated microprocessor, storage for 6 different protocols | Humidity: 10 to 90% no condensation |
| Measurement time: selectable from 1 to 999sec with 1sec steps | Recommended operating temperature: 15 to 30°C |
A test light source is available upon request.
With Life Sciences Enquiries (Luminometers, Night Owl, HPLC) please email:
| Australia & South East Asia | berthold@berthold.com.au | |
| USA | usbio@berthold.com.au | |
| Europe | eurobio@berthold.com.au |